| 2024-04-27 16:00 | J46 | Colchester |  | 1-1 |  | Crewe Alexandra | Noah Chilvers 35'; Elliott Nevitt 90' | |
| 2024-04-23 20:45 | J38 | Colchester | | 1-4 |  | Doncaster Rovers | Jayden Fevrier 9'; Luke Molyneux 14', Harrison Biggins 22', Tom Anderson 76', Joe Ironside 85' | |
| 2024-04-16 20:45 | J33 | Colchester | | 2-0 |  | Grimsby Town | Tom Hopper 5', John Akinde 79' | |
| 2024-04-09 20:45 | J36 | Colchester | | 1-2 |  | Stockport County | Cameron McGeehan 44'; Nick Powell 31', Paddy Madden 61' | |
| 2024-04-06 16:00 | J43 | Colchester | | 1-2 |  | Wrexham | John Akinde 54'; Paul Mullin 62', Max Cleworth 85' | |
| 2024-03-29 16:00 | J41 | Colchester | | 2-1 |  | Newport County | Ellis Iandolo 75', Jay Mingi 90'; Offrande Zanzala 42' | |
| 2024-03-16 16:00 | J39 | Colchester | | 1-1 |  | Walsall | Cameron McGeehan 48'; Taylor Allen 37' | |
| 2024-02-17 16:00 | J34 | Colchester | | 1-1 |  | Accrington Stanley | Noah Chilvers 40', Riley Harbottle 80' (p.p.) | |
| 2024-02-03 16:00 | J31 | Colchester | | 3-3 |  | Forest Green Rovers | Alistair Smith 65', Jayden Fevrier 67', Harry Anderson 70'; Matty Stevens 11' (p.), Christian Doidge 27', Dominic Thompson 74' | |
| 2024-01-13 16:00 | J28 | Colchester | | 1-1 |  | Bradford City | Tom Hopper 59'; Andy Cook 35' | |
| 2024-01-01 16:00 | J26 | Colchester | | 0-1 |  | Gillingham | Scott Malone 54' | |
| 2023-12-22 20:45 | J23 | Colchester | | 2-1 |  | Salford City | Joe Taylor 42', Chay Cooper 89'; Conor McAleny 90' | |
| 2023-12-09 16:00 | J21 | Colchester | | 1-2 |  | Crawley Town | Joe Taylor 90'; Liam Kelly 41', Danilo Orsi 64' (p.) | |
| 2023-12-05 20:30 | 2R | Colchester S21 | | 0-4 |  | West Ham | Dan Chesters 8', George Earthy 57', Lewis Orford 78', Divin Mubama 82' | |
| 2023-11-25 16:00 | J19 | Colchester | | 1-4 |  | Barrow AFC | Cameron McGeehan 20', Will Greenidge 52' (p.p.), Zach Mitchell 90' (p.p.); George Ray 86' 90' | |
| 2023-11-21 20:30 | Grp.D | Colchester | | 1-0 |  | Peterborough United | Bradley Ihionvien 42' | |
| 2023-11-11 16:00 | J17 | Colchester | | 1-1 |  | Sutton United | Arthur Read 80'; Omar Sowunmi 90' | |
| 2023-11-07 20:45 | J1 | Colchester | | 3-1 |  | Swindon Town | Samson Tovide 64', Noah Chilvers 90'; Jake Young 23', Liam Kinsella 49' (p.p.) | |
| 2023-10-21 16:00 | J14 | Colchester | | 1-2 |  | Harrogate Town | Chay Cooper 62'; Matt Daly 65', Samuel Folarin 80' | |
| 2023-10-07 16:00 | J12 | Colchester | | 1-3 |  | Morecambe | Cameron McGeehan 73'; JJ McKiernan 21' 53' 61' | |
| 2023-09-30 16:00 | J10 | Colchester | | 5-4 |  | Notts County | Noah Chilvers 10', Jayden Fevrier 31', Arthur Read 38' 74', Cameron McGeehan 58'; Sam Austin 21', Macaulay Langstaff 48' 90', Jodi Jones 67' | |
| 2023-09-19 20:30 | Grp.D | Colchester | | 0-5 |  | Tottenham S21 | Jamie Donley 33' 56', Jude Soonsup-Bell 53' 72', Will Lankshear 60' | |
| 2023-09-16 16:00 | J8 | Colchester | | 1-1 |  | Mansfield Town | Joe Taylor 30'; Davis Keillor-Dunn 90' | |
| 2023-09-09 16:00 | J7 | Colchester | | 2-0 |  | Tranmere Rovers | Joe Taylor 11', Bradley Ihionvien 87' | |
| 2023-08-19 16:00 | J4 | Colchester | | 2-3 |  | MK Dons | Joe Taylor 10' 13'; Mo Eisa 8', Alex Gilbey 88', Matt Dennis 90' | |
| 2023-08-15 20:45 | J3 | Colchester | | 0-2 |  | AFC Wimbledon | James Tilley 54' 85' | |
